Determining ploidy level is a key parameter for the genetic characterization of plant material in the breeding and production of plants and seeds. In the development of new varieties and cultivars, deliberate changes in ploidy level require regular monitoring of plant material.
Routine verification of ploidy as part of end-product quality control is often essential to detect spontaneous changes in ploidy level and aneuploidy. In basic botanical research, ploidy level and genome size of the material under investigation are important parameters for addressing fundamental questions in disciplines such as population biology, evolutionary biology, and systematics.
The genetic analysis of plant samples is complex – especially when it comes to speed, precision and material diversity. In practice, breeding, seed production and research face specific challenges that require reliable technologies and specialized solutions.
Common challenges:
Flow cytometry-based determination of ploidy level and genome size is a central component of modern plant analysis. It supports breeding programs, production control and scientific studies – reliably, efficiently and precisely.
Application for our solutions:
We provide precise, rapid and reliable microbiological quality control for demanding applications.
Develop new varieties and cultivars through targeted ploidy control
Quality control in animals, plants and seeds
Gain insight into genetic questions through precise DNA analysis
Efficient high-throughput analysis for large sample quantities
With our complete package consisting of reagents tailored to plant DNA analysis, instruments, software, accessories and expert support, Sysmex Partec ensures that users from industry, science and research are always well equipped.
We offer customized solutions for determining ploidy and genome size, based on over 50 years of experience in flow cytometry of plant DNA content, no matter what plant material you are working with – whether you want to screen samples in high throughput or examine a few plants for their DNA content with the highest precision.
Reagent kits for ploidy determination, including lysis buffer for nuclear extraction and staining buffer for fluorescence staining (DAPI) of nuclear DNA from plant tissues. “automate” – special package size for sample preparation in microtiter plates.
RP analyzers provide flow cytometry results on ploidy level and genome size from plant and animal material – precise, rapid, reliable and standardized.
CyFlow™ AL-30 Autoloader, in combination with CyFlow™ RP Ploidy Analyzers, enables automated, rapid and efficient analysis of large sample numbers.
CellTrics™ cell filters are used to filter plant samples, for example after nuclear extraction. Cell nuclei pass through the filter membrane, while tissue fragments are retained.
Reliable cell and particle analysis – experience you can build on
With decades of experience in cell and particle analysis, Sysmex Partec provides powerful solutions tailored to the needs of industrial applications. Our hardware and software deliver fast, precise results – directly on site in production.
Through scientifically validated methods and development in accordance with EN ISO 13485, we ensure the highest quality and reproducibility. Our adaptable, customer-specific solutions are developed in close collaboration with our clients – for maximum efficiency and accuracy.
Our international support structure and practice-oriented training programs ensure smooth implementation and sustainable use. As the only provider on the market, we offer a true end-to-end solution from a single source – from development to application.
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