Approximately 1.6 million tons of feed are fed annually to the roughly 1 million horses living in Germany. However, there is little data available on the quality of the grain used in horse feed production. This is critical because health-hazardous contaminants can be present in the feed (Liesner, 2012).
Various fungi can produce secondary metabolites such as mycotoxins (Samyal & Sharma, 2023). These are formed either in the field or later during storage. Mycotoxins are largely heat-stable and are not actively combated by the immune system (TSK BW, 2020).
In the past, various horse magazines and portals reported on the danger that mycotoxins pose to horses. (Cavallo, 2017, Cavallo, 2025, Sladky, 2016). Among other things, it was reported that horses ate feed containing mycotoxins, which consequently led to elevated liver values. According to the interviewed veterinarians, ZEN, FUM, and DON play a particular role here (Cavallo, 2025).
Research results in veterinary medicine show that mycotoxin contamination is widespread in commercially produced horse feed in Germany. Immediate severe health problems in horses usually become apparent when high amounts of mycotoxins are ingested. Little is known about the long-term consequences of ingesting moderate amounts over an extended period (Liesner, 2012).
| Mycotoxin | Occurrence | Symptoms in Horses | Sources |
|---|---|---|---|
| Aflatoxin (AFL) | Feed from warm climates, wheat | Brain, liver, kidney, and heart damage, leukocyte reduction, death | TSK BW, 2020; Janik et al., 2020 |
| Deoxynivalenol (DON) | Grain | Feed refusal, weight loss, diarrhea, gastrointestinal inflammation, immunosuppression, changes in blood and liver values | Richter et al., 2007; Liesner, 2012 |
| Fumosin (FUM) | Corn, grain | Cerebral hemorrhages and damage, severe liver degeneration, small intestine inflammation, cardiovascular problems, temperament changes, ataxias, lip and tongue paralysis, equine leukoencephalomalacia, blindness, death | TSK BW, 2020; Liesner, 2012 |
| Ochratoxin A (OTA) | Grain | Kidney diseases | TSK BW, 2020; Liesner, 2012 |
| T-2/HT-2 Toxin | Grain | Hypersalivation, skin changes, nervous system disorders | Janik et al., 2020; Liesner, 2012 |
| Zearalenone (ZEN/ZEA/ZON) | Corn, grain | Internal bleeding, hyperestrogenism, cycle disorders, death | TSK BW, 2020; Liesner, 2012 |
| Mycotoxin | Matrix | Recommended Limit (mg/kg) |
|---|---|---|
| AFL (B1) | Raw materials (hay, straw, oats) | 0.02 |
| Complementary/Complete feed | 0.01 | |
| DON | Single feed materials – Grain/products | 8 |
| Single feed materials – Maize by-products | 12 | |
| Compound feed | 5 | |
| ZEN | Grain/products | 2 |
| Maize by-products | 3 | |
| OTA | Grain/products | 0.25 |
| FUM (B1/B2) | Maize/products | 60 |
| Compound feed | 5 | |
| T-2/HT-2 | Unprocessed barley and maize | 200 |
| Oats (unhulled) | 1000 | |
| Wheat, rye, other grains | 100 | |
| Oat milling products (hulls) | 2000 | |
| Other grain products | 500 | |
| Compound feed | 250 |
Mycotoxins cannot be completely avoided. Minimizing their content through proper handling of feed is crucial for animal health. Various companies offer mycotoxin binders as feed additives. These are not recommended due to insufficient efficacy studies (Schulz & Vervuert, 2015).
To check whether horse feed is contaminated with concerning amounts of mycotoxins, laboratory analysis methods are used. A highly innovative method for the precise detection of mycotoxins is flow cytometry with a multiplex immunoassay, enabled by SAFIA technology (Suspension Array Fluorescence Immunoassay). This allows six mycotoxins (AFL, DON, ZEN, OTA, FUM, T-2) per sample to be quantified simultaneously within minutes – significantly more sensitive than the limits shown in Table 2 and at an unbeatable price!
We would be happy to explain our mycotoxin solutions in more detail: Mycotoxin Kits – Rapid Toxin Detection for Your Product Safety | Food/Animal Feed – Sysmex Partec | Flow Cytometry Made in Germany
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