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Mycotoxins, a Hidden Danger in Horse Feed

What are mycotoxins and what do they have to do with horses?

Approximately 1.6 million tons of feed are fed annually to the roughly 1 million horses living in Germany. However, there is little data available on the quality of the grain used in horse feed production. This is critical because health-hazardous contaminants can be present in the feed (Liesner, 2012).

Various fungi can produce secondary metabolites such as mycotoxins (Samyal & Sharma, 2023). These are formed either in the field or later during storage. Mycotoxins are largely heat-stable and are not actively combated by the immune system (TSK BW, 2020).

In the past, various horse magazines and portals reported on the danger that mycotoxins pose to horses. (Cavallo, 2017, Cavallo, 2025, Sladky, 2016). Among other things, it was reported that horses ate feed containing mycotoxins, which consequently led to elevated liver values. According to the interviewed veterinarians, ZEN, FUM, and DON play a particular role here (Cavallo, 2025).

Research results in veterinary medicine show that mycotoxin contamination is widespread in commercially produced horse feed in Germany. Immediate severe health problems in horses usually become apparent when high amounts of mycotoxins are ingested. Little is known about the long-term consequences of ingesting moderate amounts over an extended period (Liesner, 2012).

How exactly do mycotoxins affect horses?

Mycotoxins can lead to significant health and performance impairments in horses (Richter, et al., 2007). Corn, grain, and straw are particularly often contaminated with mycotoxins (DSM, 2023). Existing studies by various authors show significant health damage, even death, when feeding mycotoxin-contaminated feed:
Table 1: Mycotoxins in Horse Feed and Health Effects
Mycotoxin Occurrence Symptoms in Horses Sources
Aflatoxin (AFL) Feed from warm climates, wheat Brain, liver, kidney, and heart damage, leukocyte reduction, death TSK BW, 2020; Janik et al., 2020
Deoxynivalenol (DON) Grain Feed refusal, weight loss, diarrhea, gastrointestinal inflammation, immunosuppression, changes in blood and liver values Richter et al., 2007; Liesner, 2012
Fumosin (FUM) Corn, grain Cerebral hemorrhages and damage, severe liver degeneration, small intestine inflammation, cardiovascular problems, temperament changes, ataxias, lip and tongue paralysis, equine leukoencephalomalacia, blindness, death TSK BW, 2020; Liesner, 2012
Ochratoxin A (OTA) Grain Kidney diseases TSK BW, 2020; Liesner, 2012
T-2/HT-2 Toxin Grain Hypersalivation, skin changes, nervous system disorders Janik et al., 2020; Liesner, 2012
Zearalenone (ZEN/ZEA/ZON) Corn, grain Internal bleeding, hyperestrogenism, cycle disorders, death TSK BW, 2020; Liesner, 2012

What do the regulations say?

Since mycotoxins are harmful to human and animal health, regulations have been established. The European Food Safety Authority (EFSA) sets maximum levels for contaminants, such as mycotoxins, in food and feed (EFSA, 2024). In addition to EU regulations, Germany’s Contaminants Ordinance (KmV) applies to further reduce mycotoxin contamination (BfR, 2025). Thresholds at which mycotoxins have a harmful effect on horses are not yet well researched compared to other animal species (TSK BW, 2020). The Chamber of Agriculture NRW provides the following recommendations for mycotoxin limits in horse feed (LUFA NRW, 2025):
Table 2: Recommended Limits for Mycotoxins in Horse Feed – LUFA NRW (As of 2025-07-15)
Mycotoxin Matrix Recommended Limit (mg/kg)
AFL (B1) Raw materials (hay, straw, oats) 0.02
Complementary/Complete feed 0.01
DON Single feed materials – Grain/products 8
Single feed materials – Maize by-products 12
Compound feed 5
ZEN Grain/products 2
Maize by-products 3
OTA Grain/products 0.25
FUM (B1/B2) Maize/products 60
Compound feed 5
T-2/HT-2 Unprocessed barley and maize 200
Oats (unhulled) 1000
Wheat, rye, other grains 100
Oat milling products (hulls) 2000
Other grain products 500
Compound feed 250

How can horses be protected from mycotoxins?

Mycotoxins cannot be completely avoided. Minimizing their content through proper handling of feed is crucial for animal health. Various companies offer mycotoxin binders as feed additives. These are not recommended due to insufficient efficacy studies (Schulz & Vervuert, 2015).

To check whether horse feed is contaminated with concerning amounts of mycotoxins, laboratory analysis methods are used. A highly innovative method for the precise detection of mycotoxins is flow cytometry with a multiplex immunoassay, enabled by SAFIA technology (Suspension Array Fluorescence Immunoassay). This allows six mycotoxins (AFL, DON, ZEN, OTA, FUM, T-2) per sample to be quantified simultaneously within minutes – significantly more sensitive than the limits shown in Table 2 and at an unbeatable price!

We would be happy to explain our mycotoxin solutions in more detail: Mycotoxin Kits – Rapid Toxin Detection for Your Product Safety | Food/Animal Feed – Sysmex Partec | Flow Cytometry Made in Germany

ie wollen mehr zu der Entstehung und Klassifizierung von Mykotoxinen erfahren, informieren Sie sich weiter:
If you want to learn more about the formation and classification of mycotoxins, find further information here: Mycotoxin in Food and Feed, Seeds, and Fruit Juice – Sysmex Partec | Flow Cytometry Made in Germany

References and Sources

  • BfR (2025). Legal regulations on mycotoxin and plant toxin levels in food and feed. (Bundesinstitut für Risikobewertung, Ed.), bfr.bund.de, retrieved on 2025-09-10.
  • Cavallo (2017). Oats & Co: Mold is found in almost every feed (Cavallo, Ed.), cavallo.de, retrieved on 2025-09-10.
  • Cavallo (2025). Where is mold hiding in the stable? (Cavallo, Ed.), cavallo.de, retrieved on 2025-09-10.
  • DSM (2023). DSM firmenich - World Mycotoxin Survey (dsm-firmenich, Ed.), dsm-firmenich, retrieved on 2025-09-23.
  • EFSA (2024). European Food Safety Authority, efsa.europa.eu, retrieved on 2025-09-10.
  • Liesner, K. (2012). Study on the detection and occurrence of mycotoxins in horse feed. Gießen: VVB Laufersweiler, jlupub.ub.uni-giessen.de, retrieved on 2025-09-10.
  • LUFA NRW (July 15, 2025). Chamber of Agriculture NRW, landwirtschaftskammer.de, retrieved on 2025-09-23.
  • Richter, W., Schuster et al. (2007). LfL Bavaria - Molds and Mycotoxins in Feed (Bavarian State Institute for Agriculture, Ed.), lfl.bayern.de, retrieved on 2025-09-10.
  • Samyal, S., Sharma, A. (2023). Mycotoxins: Structure, Biosynthesis, Health Effects, and Their Biological Detoxification. In: Singh, I., Rajpal, V.R., Navi, S.S. (eds) Fungal Resources for Sustainable Economy. Springer, Singapore. doi.org.
  • Schulz, A., & Vervuert, I. (2015). The significance of mycotoxins in horse feeding. Der praktische Tierarzt, 96(7), 690–700, vetline.de, retrieved on 2025-09-23.
  • Sladky, P. (2016). Toxic mycotoxins widespread in feed. (pferde revue, Ed.), pferderevue.at, retrieved on 2025-09-10.
  • Tierseuchenkasse Baden-Württemberg (TSK BW) (2020). Information sheet of the Horse Health Service on mycotoxins in horse feed, tsk-bw.de, retrieved on 2025-09-10.

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